X-Cite® TURBO User Guide
X-Cite® TURBO User Guide
035-00601R rev.0
Made in Canada
Excelitas Canada Inc. 2015
All rights reserved
No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system or
translated into any language in any form by any means without the prior written consent of Excelitas
Canada Inc. Every effort has been made to ensure information in this manual is accurate; however,
information in this manual is subject to change without notice and does not represent a commitment on
the part of the authors.
Excelitas Canada Inc.
2260 Argentia Road
Mississauga ON L5N 6H7 CANADA
Trademarks
X-Cite® is a registered trademark of Excelitas Canada Inc. All rights reserved.
All other product names are trademarks of their respective owners.
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Table of Contents
1
Introduction ...............................................................................................................................5
2
Safety.........................................................................................................................................6
2.1
2.2
3
Getting Started ...........................................................................................................................8
3.1
3.2
3.3
4
Glossary of Symbols ...................................................................................................................... 6
Safety Precautions ........................................................................................................................ 6
System Components ..................................................................................................................... 8
System Layout ............................................................................................................................. 10
Installation/Set-up ...................................................................................................................... 11
Operation – Manual Control ..................................................................................................... 14
4.1
The Basics .................................................................................................................................... 14
4.2
SpeedDIAL Menu and Settings .................................................................................................... 16
4.2.1
SpeedDIAL Menu Structure................................................................................................. 16
4.2.2
LCD – Display Screen Brightness and Color Settings ........................................................... 17
4.2.3
Hand – Display Screen Orientation ..................................................................................... 17
4.2.4
Info ...................................................................................................................................... 17
4.2.5
Srvc – Service Data .............................................................................................................. 18
4.2.6
Err ........................................................................................................................................ 19
5
Operation - External Control ..................................................................................................... 19
5.1
USB/RS-232 ................................................................................................................................. 19
5.1.1
Driver Installation (automatically via internet) ................................................................... 19
5.1.2
Driver Installation (via ZIP file) ............................................................................................ 20
5.1.3
Verify Installation & Get COM Port Number....................................................................... 20
5.1.4
Software Developer’s Kit (SDK) ........................................................................................... 21
5.1.5
Commercial Software Support ............................................................................................ 21
5.1.6
Grouping Controls / Compatibility Mode ........................................................................... 21
5.2
TTL & Analog ............................................................................................................................... 22
5.2.1
TTL Input Specifications ...................................................................................................... 22
5.2.2
TTL Signal and LED Status.................................................................................................... 22
5.2.3
Analog Input Specifications................................................................................................. 22
5.2.4
Analog Signal and LED Power Setting ................................................................................. 22
5.2.5
DB25 Pin-Out....................................................................................................................... 23
5.3
Tips for Specific Use Cases .......................................................................................................... 23
5.3.1
Simultaneous Control to produce White Light Mode – USB .............................................. 23
5.3.2
Simultaneous Control to produce White Light Mode – TTL&Analog.................................. 23
5.3.3
Ring Buffer / TTL Sequencing Mode.................................................................................... 24
5.3.4
Controlling with X-Cite XLED GUI ........................................................................................ 24
6
Troubleshooting ....................................................................................................................... 24
6.1
6.2
6.3
6.4
7
Error Messages............................................................................................................................ 25
Failure to Power Up .................................................................................................................... 26
Low Illumination Intensity .......................................................................................................... 26
Other Potential Symptoms & Questions ..................................................................................... 27
Routine Care and Maintenance ................................................................................................. 27
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7.1
7.2
7.3
8
General........................................................................................................................................ 27
Cleaning - Exterior Surfaces ........................................................................................................ 27
Cleaning - Optical Surfaces ......................................................................................................... 27
Technical Specifications ............................................................................................................ 29
8.1
8.2
8.3
8.4
8.5
8.6
9
General........................................................................................................................................ 29
Electrical ...................................................................................................................................... 29
Environmental – Operating Conditions ...................................................................................... 29
Environmental – Transport and Storage Conditions................................................................... 29
Input/Output (I/O) Connections ................................................................................................. 30
Output Stability ........................................................................................................................... 30
Regulatory Compliance ............................................................................................................. 30
9.1
9.2
9.3
9.4
9.5
10
Product Safety and Electromagnetic Compatibility .................................................................... 30
CE Marking .................................................................................................................................. 31
FCC .............................................................................................................................................. 31
WEEE Directive ............................................................................................................................ 31
China RoHS .................................................................................................................................. 32
Warranty & Repairs .............................................................................................................. 32
10.1
10.2
11
Warranty Terms .......................................................................................................................... 32
Returning equipment to Excelitas............................................................................................... 33
Contact Information.............................................................................................................. 34
11.1
11.2
11.3
General........................................................................................................................................ 34
Technical Support and Service .................................................................................................... 34
Accessories and Replacement Parts ........................................................................................... 34
Table of Figures
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Front and Rear Panel Connections ................................................................................................. 9
SpeedDIAL ...................................................................................................................................... 9
LED and Dichroic Internal Layout ................................................................................................. 10
Filter bracket ................................................................................................................................ 11
Correct position for fully inserted X-Cite LLG and LLG with A and B insertion lines .................... 13
SpeedDIAL Display – Turning LED ON/OFF................................................................................... 15
SpeedDIAL Display – Adjusting Intensity...................................................................................... 15
Device Manager, COM Port Listing .............................................................................................. 21
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1 Introduction
Meet X-Cite TURBO - a powerful, color switching LED source, with six channels and excitation filtering
capabilities, for fluorescence applications. Delivering light through a liquid light guide, X-Cite TURBO
delivers superior optical power and exceptional field uniformity to the specimen with the broadest
spectrum of fluorescence excitation through manual, PC and TTL control. With LEDs rated to 15,000
hours and no lamps or modules to replace, X-Cite TURBO offers simplicity and convenience to
researchers and instrument developers, allowing them to focus on their experiments instead of
equipment maintenance.
This product is intended for use in fluorescence illumination. It allows researchers the ability to excite
fluorescence in samples being studied and characterize their location or behavior.
Excelitas Technologies Corp. is a global technology leader focused on delivering innovative, customized
solutions to meet the lighting, detection and other high-performance technology needs of customers. XCite, formerly of Lumen Dynamics (which was acquired by Excelitas Technologies Corp. in November
2013) offers the Life Science and Analytical Instrumentation market a broad range of innovative lamp
and LED fluorescence illumination and measurement solutions. Recognized as the industry standard in
research microscopy, X-Cite also provides solid state LED technologies to instrument manufacturers, and
combines maximum optical performance with flexible design, as well as best-in-class manufacturing
quality and lead time.
Excelitas recommends reading this manual to discover all the features available in X-Cite TURBO.
Thank you for choosing X-Cite!
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2 Safety
2.1 Glossary of Symbols
Symbol
Meaning
CAUTION - Risk of danger: consult accompanying documents
WARNING – Eye damage may result from directly viewing
ultraviolet light. Protective eye shielding and clothing must
be used at all times.
WARNING – UV light hazard.
WARNING – Class 1 laser product.
IEC 60825-1: Safety of laser products –
Part 1: Equipment classification and
requirements: 2014-05
Input/Output Signals
Input Signal
CAUTION – Hot surface
2.2 Safety Precautions
Please observe the following safety precautions at all times during operation and maintenance of this
product. Failure to do so may result in personal injury or property damage.
1. UV emitted from this product. Avoid eye and skin exposure to unshielded product. Do not look
at operating lamp/LED. Eye injury may result.
2. Never look into the light emitting end of the light guide. The light could severely damage the
cornea and retina of the eye if the light is observed directly. Eye shielding must be used at all
times as well as clothing to protect exposed skin.
3. Always make sure the light guide and microscope adapter (if applicable) are securely attached to
the microscope prior to turning on power to the unit. This will minimize the risk of exposure to
the UV light.
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4. Always use this product with the external power supply and power cord set provided. Ensure
the external power supply cord is connected to a grounded 3-pin outlet only. Any substitution of
these components will invalidate regulatory certification of this product and may impair
operating safety.
5. Disconnecting of main supply source is done by putting the on/off button in the off position and
unplugging the power cord.
6. It is recommended that ONLY QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs
of this unit. If servicing the unit, disconnect the external power supply before opening the
cover of this unit. All cover screws must be replaced prior to applying power to the unit, or
safety of the unit will be impaired. Note that opening the cover and/or altering the unit in any
way may void the warranty (see section 10 for more details).
7. Monitoring the unit during manual operation
The level of UV and visible energy supplied by this product is sufficient to ignite flammable
substances. During manual operation, the unit must be attended at all times by a qualified
operator. The unit must not be left unattended while turned on. If an operator leaves the work
area of the unit, the power switch must be turned off.
8. Monitoring the unit during automated operation
The level of UV and visible energy supplied by this product is sufficient to ignite flammable
substances. Therefore, when the unit is operated unattended in an automated environment, an
alarm function must be provided by the user to indicate a malfunction in the associated
equipment used.
9. Should this X-Cite unit be used in a manner not specified by Excelitas, the protection provided
by the equipment may be impaired.
10. This unit is designed for bench top use only! Always ensure that the unit is placed on a hard,
stable surface, ensuring that ventilation openings are not obstructed. Any obstruction of these
openings could result in a possible over-heating condition.
11. To clean the exterior of the unit, use a slightly dampened cloth and a simple water/ detergent
solution only. Avoid the optical surfaces and lenses. Cleaning of optics should only be attempted
by qualified personnel using appropriate fluids and lens paper.
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3 Getting Started
3.1 System Components
This X-Cite system contains the following components:
1.
2.
3.
4.
5.
X-Cite TURBO unit (Model no: XT600-T), LEDs and dichroics are installed
SpeedDIAL Manual Controller
External Power Supply and Power Cord
Microscope Adaptor (if ordered)
Accessories Box, containing:
a. Quick Start Instruction Sheet (User Guide and Software download are available on
Excelitas website: http://www.excelitas.com/Pages/Product/X-Cite-TURBO.aspx)
b. Hex Tools (Allen Keys), 3mm and 0.05 inch
c. Set screws (6) for filter brackets
d. USB Cable
e. Light Guide
f. TTL/Analog Trigger Cable, DB25 to 12 x BNC
If any components are missing or appear damaged, please contact Excelitas immediately.
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Filter
Access
(on side)
TTL / Analog
USB
SpeedDIAL
Light
Guide
Port
ON/OFF
Power
(DC Input)
Figure 1 Front and Rear Panel Connections
Rotating Dial
Display Screen (LCD)
“up” / “down”
buttons
Figure 2 SpeedDIAL
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3.2 System Layout
X-Cite TURBO unit contains the following LED wavelengths:
LED Position
1
2
3
4
5
6
LED Center Wavelength
385nm
430nm
475nm
525nm
575nm
630nm
Useable Wavelength Range
375nm – 400nm
410nm – 450nm
460nm – 495nm
500nm – 520nm
525nm – 610nm
615nm – 660nm
The LEDs (black numbers) and the dichroics (white numbers) that combine them are positioned as
shown in Figure 3.
Figure 3 LED and Dichroic Internal Layout
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3.3 Installation/Set-up
1. Unpack
a. Carefully unpack the unit and accessories from the shipping carton.
b. When unpacking the light guide (liquid or quartz fiber), take care not to bend sharply or
kink.
2. Installing Excitation Filters (if using)
Note: 25mm mounted Excitation filters are not supplied with the system.
Important: Ensure that main unit is powered OFF before attempting to install filters
a. Remove the filter access panel with the 3mm hex tool. Note that the access panel is
holding the filter brackets in place – if work surface is not level, the brackets may begin
to slide out on their pins once the panel is removed. You may prefer to lay the system
on its side for filter installation/exchange.
b. Refer to Figure 3 to determine location of specific LEDs.
c. Slide out the relevant filter brackets by grasping the black “tabs”.
d. Place 25mm mounted filter in the bracket in the correct orientation advised by the filter
manufacturer.
e. Tighten a set screw at the bottom of the bracket.
f. Over a clean surface, turn filter bracket over to ensure the filter is firmly installed.
g. Repeat for any additional filters being installed.
h. Reinstall the filter bracket(s) by aligning the holes with the locator pins, and gently
sliding the bracket all the way in.
i. Reattach the filter access panel.
Tab
Allen key
Set screws
Figure 4 Filter bracket
3. Install the Microscope Adaptor (if applicable)
a. Remove the protective wrap from the Microscope Adaptor, being careful not to touch
the lens surfaces.
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a. Insert the flange portion of the Microscope Adaptor into the light port on the
microscope, and secure it using the hardware provided on the microscope. (Refer to
relevant microscope user manual for complete instructions on mounting a standard epifluorescence lamphouse.) General guidelines:
i. Carl Zeiss – Tighten the hex fastener on the side of the light port with the 3mm
Hex Tool.
ii. Leica - Tighten the hex fastener on the side of the light port with the 3mm Hex
Tool.
iii. Nikon – Line up the “notch” with the pin on the microscope fitting, hold the
adaptor firmly against the light port, and twist the collar to lock the components
together.
iv. Olympus - Tighten the hex fastener(s) with the 3mm Hex Tool. Depending on
the model, there may be one (1) fastener on the side, or two (2) fasteners
located at the 10:00 and 2:00 positions.
4. Install the Light Guide
a. Remove the red plug from the light guide port located on front fascia.
b. Remove the red caps from the ends of the light guide.
c. Insert the larger end of the light guide into the light guide port. For X-Cite TURBO, the
light guide must be inserted up to the “B” line printed on the grey ferrule, see Figure 5.
[Note: If using an X-Cite 3mm or 5mm light guide with only one “insertion line” on the
grey ferrule, this line will be 20mm away from the light guide port.]
d. Insert smaller end of the light guide into the Microscope Adaptor (or light guide port on
the microscope/imaging instrument). Ensure that light guide is fully inserted and lock in
place with thumbscrew (or appropriate fastener).
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Figure 5 Correct position for fully inserted X-Cite LLG and LLG with A and B insertion lines
5. Check Position of the Equipment
a. The X-Cite unit should be positioned to avoid sharp bends and strain on the light guide.
This applies to both liquid and quartz fiber light guides. Ideally, the unit should be
positioned so that:
i. The light guide has as few bends as possible.
ii. Bends have the largest bend radius possible.
iii. There is some slack in the light guide.
b. To avoid overheating the LEDs and other electronics, ensure all air vents on the side
panel of the unit are clear of obstruction and have ready access to room temperature
air. Recommended clearance is 20cm (8 inches).
c. If installing the unit within another imaging instrument, the air vent side should be
placed against an outside panel with venting to allow fresh air to be drawn into the
system.
d. Note that the main power switch is located on the rear panel of the unit. The unit
should be placed so that the switch is convenient for the operator to access.
6. Connect speedDIAL to main unit
a. Important: Ensure that main unit is powered OFF before attempting to connect
speedDIAL, or damage to the device may result.
b. Insert the mini DIN plug into the “speedDIAL” port on the rear of the unit. Ensure the
arrow mark on the connector is on the right (i.e. at the 3:00 position). Note: Never
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force the connector – this can damage the pins. If connector does not insert smoothly,
stop and check for bent pins.
c. Place speedDIAL next to the microscope, or another easily accessible location.
7. Connect USB (if using)
a. Insert “B” (square) end into the “USB” port on rear of the unit.
b. Insert “A” (flat) end into an available port on the computer.
c. Note: For best performance, use the supplied USB cable (or one of equivalent quality
and length), and plug directly into the computer. Using a USB hub or longer USB cable
than the one supplied may result in intermittent communication errors.
8. Connect TTL/Analog Trigger Cable (if using)
a. Insert DB25 end into the port on rear of the unit. Tighten fasteners with flat head
screwdriver.
b. Connect BNC cables to appropriate signal generating device. BNC connectors are
labeled with TTL and Analog channel numbers.
9. Connect AC Power
a. Connect Power Supply: Insert the 4-pin connector on the cable into the power port on
rear of the main unit. Ensure that the orientation is correct, or else damage to the pins
may result. The arrows on the plug should be on the right (i.e. at the 3:00 position).
b. Connect Power Cord: Connect female end of the power cord to AC port on the power
supply. Connect male end to a properly grounded electrical outlet.
c. For safe operation, use only the power cord supplied or one with an equivalent rating.
4 Operation – Manual Control
4.1 The Basics
1. Start the unit
a. Flip the rocker switch on rear of the main unit to turn X-Cite system on.
b. The system will have a brief initialization period (approximately 5 seconds). The
speedDIAL display will show “X-Cite” during this time.
c. When the display shows LED positions 1 and 2 with their intensity setting (a number
from 5 to 100 for LEDs 1, 2, 3, 4 and 6; a number from 10 to 100 for LED 5), it is ready to
use.
d. Note: If speedDIAL will be used for manual control, it must be connected before turning
system on. SpeedDIAL can be damaged if it is plugged into, or unplugged from, the
system while it is powered on.
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2. Illuminate a specimen
a. Press the “up” and “down” buttons until the arrow is pointing to the desired LED
channel (1 to 6).
b. Click the dial (shutter button) on speedDIAL to turn on/off the excitation light. A dot will
appear beside the LED channel numbers that are ON. (See Figure 6 – LED 1 is off, LED 2
is on at 100% intensity.)
c. To turn all LEDs ON/OFF at once, move arrow to “All” at the bottom of the list. Click the
dial. Note: All function is controlled from speedDIAL, if using the command line all
on/off control, the speedDIAL all on/off status will not correlate.
Figure 6 speedDIAL Display – Turning LED ON/OFF
3. Adjust intensity
a. Press the “up” and “down” buttons to the LED to be adjusted.
b. Turn the dial to adjust intensity - clockwise to increase, counter-clockwise to decrease.
intensity is updated in real time) (See Figure 7 – LEDs 1 and 2 are both OFF, LED 1 is set
to 100%, LED 2 is being set to 42%)
c. Repeat steps b and c for any other LEDs requiring adjustment.
d. The dial is speed sensitive – turning slowly will allow adjustments in smaller increments,
turning quickly will increase the step sizes.
e. Intensity can be adjusted in 1% increments from 5% to 100%. (Note: 5% is the minimum
for LED 1, 2, 3, 4 and 6; 10% is the minimum for LED 5)
Figure 7 SpeedDIAL Display – Adjusting Intensity
NOTE: If the LEDs are Locked via PC, the “Lock” symbol will show to the right of “All”. Unit can only be
unlocked via PC control, or by power cycling the unit.
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4.2 SpeedDIAL Menu and Settings
In addition to the intuitive intensity adjustment and illumination ON/OFF control, speedDIAL has several
advanced settings and control options.
To access the main menu, press and hold the dial for one (1) second.
To navigate the menus, turn the dial to scroll through the options. An arrowhead will indicate
the currently selected menu option; click the dial to make a selection.
To adjust settings, turn the dial. To exit the setting adjustment, click the dial.
To exit the menu system at any time, press and hold the dial for one (1) second, or select Exit at
the bottom of the menu list.
Note: Setting changes will take effect immediately during selection. However, for the first five (5)
minutes, the new settings are not saved for restoration after power cycle. If the X-Cite unit is powered
down during this time, settings will revert back to their previous values. To ensure that new settings will
be remembered, wait at least five (5) minutes after the last settings adjustment before powering down
the unit. “Up” and “down” buttons are disabled in the speedDIAL menu and settings.
4.2.1 SpeedDIAL Menu Structure
LCD
Hand
Info
Dim
Brig
Color
Exit
LED1
o
Hour, Wave, FWH, WLR,
Temp, Max
LED2 to LED6
(same as LED1)
Exit
Srvc
SN
S/W1
S/W2
Spd (no function in X-Cite Turbo)
Exit
Err
Exit
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4.2.2 LCD – Display Screen Brightness and Color Settings
In the LCD sub menu, the time before the backlight is turned off (the backlight will turn back on
with a movement of the speedDIAL), the backlight on the display screen can be turned on/off,
set to a different brightness level, or set to a different color.
a. Select the “LCD” option from main menu.
b. To adjust LCD display backlight time out:
i. Select “Dim” and scroll through the time out options, which are: Off and 1 thru
999 seconds in 1 second increments. “Off” will turn off the backlight timer (i.e.
backlight will always be on).
ii. Click dial to return to “LCD” menu.
c. To adjust LCD brightness:
i. Select “Brig” and scroll through % brightness settings until the desired level is
reached. To turn backlight off, set level to 0%.
ii. Click dial to return to “LCD” menu.
d. To change LCD color:
i. Select “Color” and scroll up /down through the options until desired color is on
LCD.
ii. Click dial to return to “LCD” menu.
e. Select “Exit” to go back to the main menu, or press and hold dial to return to the home
screen.
4.2.3 Hand – Display Screen Orientation
In this menu, the LCD orientation can be rotated 180°. This allows operators to use speedDIAL
on the left or right side of the microscope without blocking the view of the display with their
hand.
a. Select “Hand” option from main menu.
b. Turn dial to toggle between “Right Hand” and “Left Hand” operation. Note that the
screen will flip while adjusting this setting.
c. Click dial to return to the main menu.
4.2.4 Info
In this menu, information specific to each LED may be found. This includes total hours
accumulated on the LED, center wavelength, full width half maximum of each LED, useable
wavelength range and temperature.
a. Select “Info” option from the main menu.
b. Scroll through the LED list and click on the “LED#” of interest.
c. To obtain the LED “in use” hours:
i. Select the “Hour” menu option.
ii. LED “hours of use” will be shown in one (1) hour increments from 0 to 999
hours. Due to space limitations on the LCD, when 1000 hours are logged, the
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d.
e.
f.
g.
h.
i.
format will change to “1.0k hours”, and increments will increase to 100 hours
(e.g. 1142 hours will display as “1.1k hours”). The precise hour-by-hour data will
continue to be available via USB communication.
iii. Click dial to return to previous sub menu.
To obtain center wavelength information:
i. Select the “Wave” menu option.
ii. Center wavelength will be shown as “###”, units are nanometres.
iii. Click dial to return to previous sub menu
To obtain full width half maximum information:
i. Select the “FWH” menu option.
ii. Width will be shown as “##”, units are nanometres.
iii. Click dial to return to previous sub menu.
To obtain wavelength range information:
i. Select the “WLR” menu option.
ii. Due to space limitations on the LCD, wavelength range will be shown as two
alternating values “###-” and “###”. The delay between screen views is
approximately one second. Units are nanometres.
iii. Click dial to return to previous sub menu.
To obtain current LED temperature:
i. Select the “Temp” menu option.
ii. Current temperature will be shown in degrees Celsius.
iii. Click dial to return to previous sub menu.
To obtain maximum allowable LED temperature:
i. Select the “Max” menu option.
ii. Maximum allow allowable temperature will be shown in degrees Celsius. (Note:
should the “Temp” value ever equal or exceed the “Max” value during
operation, the LED will be turned off and an Error condition will result.)
iii. Click dial to return to previous sub menu.
Select “Exit” to go back to the main menu, or press and hold dial to return to the home
screen.
4.2.5 Srvc – Service Data
In the Service menu, information specific to each X-Cite unit can be found. This includes unit
serial number, embedded software versions for main unit and speedDIAL. Tech Support
personnel may request some or all of this information during a support call.
a. Select “Srvc” option from main menu.
b. To obtain the unit serial number:
i. Select the “SN” menu option.
ii. Serial number of the unit will be shown.
iii. Click dial to return to “Srvc” menu.
c. To obtain the embedded software version numbers:
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i. Select the “S/W1” or “S/W2” menu items. The software version numbers will be
shown in the format X.X.X.
ii. Click dial to return to “Srvc” menu.
d. “Spd” – no function in X-Cite Turbo
4.2.6 Err
In the Error menu, error conditions specific to each LED or the system in general can be checked.
Tech Support personnel may request some or all of this information during a support call.
a. Select “Err” option from main menu.
b. Scroll to find the “LED#” of interest or “Sys” for the system/unit in general. Click to
select.
c. A numerical error code will be displayed. If no error condition is present, “0” will be
displayed. Refer to section 6.1 for definition of the error code numbers.
d. Click dial to return to previous sub menu.
e. Select “Exit” to go back to the main menu, or press and hold dial to return to the home
screen.
5 Operation - External Control
5.1 USB/RS-232
This X-Cite unit is a USB device which operates at USB 2.0 speeds of 12Mb/s and does not use a USB to
RS-232 adapter. However, the X-Cite will appear as a COM port in Device Manager, and is functional as
a COM port for programming purposes. This is done for legacy program support and ease of integration.
A virtual COM port driver must be installed to enable Windows communication via the USB port. For
computers running Windows which are connected to the internet (and running an OS that Microsoft
supports windows updates for), the driver will install automatically. For manual installation, the driver
is available as a software download from the Excelitas website. Note that administrator privileges may
be required to install drivers on your computer, in which case you may need to contact your IT
department for assistance.
5.1.1 Driver Installation (automatically via internet)
For these instructions: internet is required, Windows 7 prompts are listed
a.
b.
c.
d.
e.
Ensure X-Cite unit is powered off.
Ensure X-Cite unit is connected to the computer with the USB cable.
Ensure computer is connected to internet.
Power on X-Cite unit.
Driver installation will begin automatically. A dialogue box will confirm installation has
started.
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f.
i. If the dialogue box reports that Driver installation was not successful, click on
Get Details.
ii. Click on option to Change Settings to Automatically search Windows for
drivers. Confirm Yes, do this automatically, and save changes.
A second dialogue box will confirm successful installation and provide a COM port
number. Note the COM port number for use in other software applications.
5.1.2 Driver Installation (via ZIP file)
For these instructions: download the driver ZIP file from the Excelitas website
http://www.excelitas.com/Pages/Product/X-Cite-TURBO.aspx
a. Ensure X-Cite unit is powered off.
b. Ensure X-Cite unit is connected to the computer with the USB cable.
c. Prepare the driver files by extracting and saving the files into a folder on desktop (or
another easily accessible location).
d. Power on X-Cite unit.
e. The “new hardware found” wizard will appear. Select No to search Windows update for
the software. Click Next to continue.
f. Select Install from a list or specific location (Advanced) to locate the driver and click
Next. Select Include this location in the search: and browse to the location on your
hard drive where unzipped files were saved in step c. Click Next.
g. Wait for installation to complete. Click Finish.
5.1.3 Verify Installation & Get COM Port Number
a. Open the “Device Manager” utility on the computer.
b. “X-Cite LED (XT600 Series)” will be listed under “Ports (COM & LPT)”.
c. Note the COM port number for use in other software applications.
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Figure 8 Device Manager, COM Port Listing
5.1.4 Software Developer’s Kit (SDK)
The command list is available by request. To obtain the latest update, please contact Technical
Support. Tips for specific use cases can be found in section 5.3.
5.1.5 Commercial Software Support
a. This X-Cite unit can be controlled through several commercially available packages.
Where model-specific control may not yet be available, basic functionality can be
achieved using X-Cite XLED1 hardware drivers and “Grouping” – see next section. For an
updated list of software packages that support X-Cite products, see
http://www.excelitas.com/Downloads/XCite-softwareSupport.pdf
b. For commercial software packages, if requested specify the serial port parameters as:
19,200 baud, no parity, 8 data bits and 1 stop bit.
c. When controlling this X-Cite unit, some commercial software packages may block
manual input from speedDIAL. The lock icon will appear on the speedDIAL display in
these cases.
5.1.6 Grouping Controls / Compatibility Mode
To enable compatibility with existing device drivers for X-Cite XLED1, it is possible to run X-Cite
TURBO in 4-channel mode by creating “Groups”. Groups can be configured and enabled using
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the LED Grouping and Trigger Sequencing commands in the SDK. Up to four groups can be
defined, and each group may contain 1-6 LEDs. See table below for an example.
Group Number
1
2
3
4
LEDs in Group
1 – 385nm
2 – 430nm
3 – 475nm & 4 – 525nm
5 – 575nm & 6 – 630nm
5.2 TTL & Analog
5.2.1 TTL Input Specifications
a.
b.
c.
d.
e.
Connector type: BD25 (female)
Maximum low level : +0.8V
Minimum high level : +2.2V
Maximum high level: + 5.5V
Typical input current: 800µA
5.2.2 TTL Signal and LED Status
TTL
High
Low
LED Status
ON
OFF
5.2.3 Analog Input Specifications
a. Connector type: DB25 (female port)
b. Maximum input current: 500µA
5.2.4 Analog Signal and LED Power Setting
Analog Signal
0-1V
1-1.2V / 1-1.4V
1.2- 5V for LED 1-4 & 6 / 1.4-5V for LED 5
5-5.25V
LED Power Setting
Signal ignored*
5% for LED 1-4 & 6 / 10% for LED 5
Conversion factor: 0.04/1%
100%
*Note: If input voltage is below 1V, analog input will be ignored. When LED is triggered it will turn on
with previous power setting or with USB defined power setting. If an analog input is provided along with
input from speedDIAL or USB, the analog input will take precedence. Analog intensity settings are not
saved to the unit and will not be restored after a power cycle.
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5.2.5 DB25 Pin-Out
Pin Number
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Channel
1
1
2
2
3
3
4
4
5
5
6
6
1
1
2
2
3
3
4
4
5
5
6
6
Description
TTL Input
IsoDGND
TTL Input
IsoDGND
TTL Input
IsoDGND
TTL Input
IsoDGND
TTL Input
IsoDGND
TTL Input
IsoDGND
Reserved – Do not connect
Intensity Input
IsoAGND
Intensity Input
IsoAGND
Intensity Input
IsoAGND
Intensity Input
IsoAGND
Intensity Input
IsoAGND
Intensity Input
IsoAGND
5.3 Tips for Specific Use Cases
Refer to the SDK for full programming and command details.
5.3.1 Simultaneous Control to produce White Light Mode – USB
To simultaneously control all six LED channels with single commands:
a. ON/OFF - Use the “turn all LEDs ON/OFF” command.
b. Intensity Setting – use the standard intensity setting command, and set all six LED
intensities in a single string (#, #, #, #, #, #). All six must be given a % value * 10 (a # of
500 sets the intensity to 50.0%) - so you can use the same setting for all channels, or
different settings to remove the UV component or otherwise adjust the color balance.
c. Note - Refer to SDK for complete syntax details.
5.3.2 Simultaneous Control to produce White Light Mode – TTL & Analog
To simultaneously control all six LED channels with a single TTL input:
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a. ON/OFF - Configure a TTL Trigger Sequence where all LEDs are set to “1” via a USB
command. Connect triggering device to input TTL1. (Please see SDK)
b. Intensity Setting
a. USB - If intensity does not need to change constantly, use the standard intensity
setting command, and set all six LED intensities in a single string (#, #, #, #, #, #).
All six must be given a % value * 10 (a # of 500 sets the intensity to 50.0%) - so
you can use the same setting for all channels, or different settings to remove
the UV component or otherwise adjust the color balance.
b. Analog – If intensity needs to be adjusted during imaging session (without a
USB connection), set analog input to “Analog1” For this to be applicable, a USB
command to use one analog input also needs to be given. (Please see SDK)
5.3.3 Ring Buffer / TTL Sequencing Mode
To control all six LED channels with a single TTL input, there is a one-time set-up with USB
commands. After five minutes, the settings will be stored in NVM and do not need to be
adjusted until new settings are desired. Use TTL input channel TTL1 to trigger.
To configure TTL ON/OFF settings:
a. Via USB commands - enable TTL sequencing and triggering order with the “ts”
command. See SDK for syntax details.
5.3.4 Controlling with X-Cite XLED GUI
For demonstration, communication testing purposes and existing software compatibility
purposes, X-Cite TURBO can emulate the XLED command set and be controlled with an X-Cite
XLED GUI.
a. Configure LED Groups and enable Compatibility Mode on X-Cite TURBO via USB. This can
be done via HyperTerminal using SDK commands. Refer to section 5.1.6 for complete
details.
b. Install the XLED GUI (Control Panel Software: 032-00136.zip file) from Excelitas website:
http://www.excelitas.com/Pages/Product/X-Cite-XLED1.aspx
(Note: XLED GUI versions prior to 1.1.0.2, including the one available for download on
the X-Cite website will not be compatible with X-Cite TURBO.)
c. Open XLED GUI and use as normal.
6 Troubleshooting
Organized by symptom, this section provides basic troubleshooting information for installation and setup parameters. X-Cite TURBO may be serviced by authorized technical personnel only.
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6.1 Error Messages
If X-Cite TURBO detects a problem, an error message with one of the following codes will appear on the
speedDIAL display.
LED Errors
Error Code
Description
1
An LED has exceeded
maximum operating
temperature
2 or 4
Laser Protection Error
3 or 5
Internal Error
6
An LED is below minimum
operating temperature
Action
Turn off the system and wait for the LED to cool
down. Verify that the system has been installed with
adequate clearance for ventilation, especially around
vents on the side panel of the unit. If fan is not
running or the problem persists contact technical
support for assistance.
Power cycle the X-Cite TURBO unit. If the error
message reappears contact technical support for
assistance.
Power cycle the X-Cite TURBO unit. If the error
message reappears contact technical support for
assistance.
Ensure the room temperature is within the
recommended operating limits. If unit has been
stored / transported in a cold environment, allow it to
warm up to room temperature and restart. If the
problem persists contact technical support for
assistance.
System (Sys) Errors
Error Code
Description
1
Sensor Input Error
3
Internal Error
5
Inadequate System
Performance
6
Low Power Error
Action
Turn off the system and wait for the LED to cool
down. Verify that the system has been installed with
adequate clearance for ventilation, especially around
vents on the side panel of the unit. If fan is not
running or the problem persists contact technical
support for assistance.
Power cycle the X-Cite TURBO unit. If the error
message reappears contact technical support for
assistance.
Reduce the rate at which commands are given to the
unit as it is unable to keep up with the commands.
The system experienced a brown out and had to shut
down the LED’s as a safety precaution. Ensure the
unit is given constant power or contact service if
problem persists.
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6.2 Failure to Power Up
If X-Cite TURBO fails to power up or function properly, use the following checklist to eliminate the most
common causes of problems. Check for the following:
1. Power Connection Check:
a. The power supply cord is securely connected to a grounded (earthed mains socket)
functional outlet.
b. The power supply cord is securely connected into the 3-pin outlet on the power supply
brick
c. The DC cord from the power supply is securely connected to the “Power” port on the
rear of the X-Cite unit.
d. The main power switch is in the ON position.
2. SpeedDIAL Check:
Verify that the pins on speedDIAL connector are straight.
Verify that speedDIAL is plugged securely into main unit.
3. Unit door check:
Verify the door screws of main unit are tightened
6.3 Low Illumination Intensity
1. SpeedDIAL Setting Check:
a. Verify that the LED intensity is set to a sufficient level.
b. Verify that the LEDs are turning on.
2. Light Guide Check:
a. Verify that the ends of the light guide are free of debris.
b. Verify that there are no bubbles in the light guide. (For detailed steps see:
http://www.excelitas.com/Downloads/XCite-120-TroubleShooting.pdf section 2.2.)
c. Verify that the light guide is fully inserted in the main unit and the microscope.
3. Microscope Check:
a. Verify that the Microscope Adaptor is appropriate for the microscope configuration.
Note: Some adaptors have the same mechanical fitting but different optics.
b. Verify that everything in the microscope beam path is properly aligned and open, e.g.
shutters, apertures, diaphragms, filters, filter cubes, etc.
c. Verify that microscope filter sets are for the appropriate wavelengths.
d. Verify that air objectives are clean / immersion objectives have enough of the
appropriate fluid.
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6.4 Other Potential Symptoms & Questions
Category
speedDIAL
Symptom
New settings are forgotten when
unit is shut down and powered up
Turning dial does not scroll through
the menu options
“NO LG” icon is on display
“Lock” icon is on display, speedDIAL
not responding to manual control
Output Linearity
Plotting output power vs. intensity
setting is not a straight line
Action
After changing settings, wait at least five (5)
minutes before shutting unit down.
Turn dial in the opposite direction.
Ensure the light guide is fully inserted in the
main unit
Send “unlock” command via computer OR
power down unit and restart.
No action. “x%” shown on speedDIAL refers
to LED input current, which is not
necessarily linear with optical output.
7 Routine Care and Maintenance
7.1 General
X-Cite TURBO is a very low maintenance system with only one consumable component – the liquid light
guide. By maintaining the following conditions, performance will be maximized and risk of future
problems will be reduced.
1. Maintain a clean work area, keeping the X-Cite TURBO air vents unobstructed.
2. Ensure that the light guide has some slack in it and is never cut, stretched, kinked, or forced into
a sharp bend.
3. Never touch optical surfaces with fingers, tools or any other abrasive/sticky/sharp materials or
fluids.
4. If cleaning is required, follow the directions in the next section.
7.2 Cleaning - Exterior Surfaces
If necessary, exterior surfaces of X-Cite TURBO and speedDIAL can be cleaned with a mild soap and
water solution and lint-free cloth.
1.
2.
3.
4.
Turn unit off and disconnect AC power prior to cleaning.
Use a damp cloth only - do not allow cleaning solution to get into I/O ports, air vents or seams.
Avoid optical surfaces.
Allow the unit to dry before turning it on.
7.3 Cleaning - Optical Surfaces
Cleaning of optical surfaces is not generally required. However, if any visible contamination or
fingerprints appear on the lens surface, cleaning may be necessary.
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1. Recommended Cleaning Materials
a. Rubber bulb dust blower
b. Lint-free lens tissue, lint-free cotton swabs
c. Powder-free gloves or finger cots
d. Lens cleaning solution, reagent-grade isopropyl alcohol, or another appropriate solvent
2. Cleaning Procedure
a. Use rubber dust blower to blow off any loose lint, dust or other contaminant.
b. If the contaminant is a fluid (e.g. water, immersion oil), first use a dry lens tissue (or
cotton swab) to wick away as much as possible – do not wipe.
c. Saturate a corner of the lens tissue (or the cotton swab) with cleaning solvent, and
gently wipe the optical surface in one pass. Note: Take care not to “flood” the area
with solvent, particularly near unsealed joints (e.g. lens-retaining ring interface).
d. Repeat the previous step with a fresh portion of lens tissue (or new cotton swab) – this
will help avoid recontamination of the optical surface and minimize the amount of
cleaning required.
e. Let solvent evaporate and verify that the optical surface is clean. Repeat cleaning steps
as necessary.
f. Before reinstalling and/or using the optics, allow them to dry completely.
CAUTION: Before using any solvent, consult the manufacturer’s Material Safety Data Sheet (MSDS)
and your internal Health and Safety Advisor for proper handling, storage, and disposal instructions.
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8 Technical Specifications
8.1 General
Main Unit
264mm (10.4″)
110mm (4.3″) a
230mm (9″)b
4.3kg (9.4lbs)
speedDIAL
59mm (2.3″)
80mm (3.1″)
112mm (4.4″)
0.3kg (0.7lbs)
Height
Width
Depth
Weight
Notes:
a. Does not include clearance required for ventilation.
b. Does not include clearance required for cables or light guide.
8.2 Electrical
AC input information for external power supply:
Power Supply
Power Factor Corrected, Universal Input
Input Voltage
100-240VAC, 50/60Hz
Current
1.85A max /115V, 1.0A max/230V
Input Surge
With cold start, 120A max/230V
Protection
Over load and over temperature
DC input information for X-Cite TURBO:
Model #
Input Voltage
XT600-T
24VDC
Input Current
6.5A
8.3 Environmental – Operating Conditions
Ambient Temperature
Altitude
Atmospheric Pressure
Relative Humidity
Installation Category
Pollution Degree
10° to 30° C
2000m max
700 to 1060 hPa
15 to 90% RH (non-condensing)
II
2
8.4 Environmental – Transport and Storage Conditions
Temperature
Relative Humidity
Atmospheric Pressure
-35° to 60° C
10 to 95% RH (non-condensing)
500 to 1060 hPa
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8.5 Input/Output (I/O) Connections
Connection
Remote - IN/OUT
Connection Style
Mini DIN plug, 9pos
USB - IN/OUT
B
Trigger Input
DB25
Purpose
Communication between speedDIAL and
X-Cite unit to control LED and report
status (on/off, intensity adjustment,
system error, etc.)
Communication between computer and
X-Cite unit to control LED and report
status.
External trigger to turn LEDs on/off and
adjust LED intensities
8.6 Output Stability
High power LEDs tend to lose their output stability when operated at low drive currents (i.e. set to low
intensity levels). To prevent power fluctuations, X-Cite TURBO has a built-in 5% lower limit on the
intensity setting for LEDs 1, 2, 3, 4, 6 and 10% lower limit for LED 5. To further maximize output stability
if an application requires lower power levels, strategies to avoid fluctuations include:
Increase power level, and reduce exposure time to compensate for brighter signal.
Increase power level, and use a neutral density filter or iris in the microscope light train to
reduce intensity to an appropriate level for the specimen.
9 Regulatory Compliance
9.1 Product Safety and Electromagnetic Compatibility
The X-Cite TURBO has been tested and found to comply with product safety and electromagnetic
compatibility requirements. For a complete list of tests and for certification details, please contact your
X-Cite representative or visit www.excelitas.com/x-cite.
The X-Cite TURBO is classified as Class 1 laser product. IEC 60825-1: Safety of laser products – Part 1:
Equipment classification and requirements: 2014-05. This class is eye-safe under all operating
conditions. This class includes all lasers or laser systems which cannot emit levels of optical radiation
above the exposure limits for the eye under any exposure conditions inherent in the design of the laser
product. There may be a more hazardous laser embedded in the enclosure of a Class 1 product, but no
harmful radiation can escape the enclosure.
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9.2 CE Marking
Council Directive 2006/95/EC
Council Directive 2004/108/EC
Council Directive 2012/19/EU
Council Directive 2011/65/EU
Low Voltage Directive
EMC Directive
WEEE Directive
RoHS
9.3 FCC
FCC Class A Digital Device or Peripheral - Information to User
NOTE
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This equipment
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance
with the instruction manual, may cause harmful interference to radio communications. Operation of this
equipment in a residential area is likely to cause harmful interference in which case the user will be
required to correct the interference at his/her own expense.
WARNING
Changes or modifications not expressly approved by Excelitas could void the user’s authority to operate
the equipment.
9.4 WEEE Directive
The symbol above indicates that this product should not be disposed of along with municipal waste, that
the product should be collected separately, and that a separate collection system exists for all products
that contain this symbol within member states of the European Union.
The equipment that you bought has required the extraction and use of natural resources for its
production. It may contain hazardous substances that could impact health and the environment.
In order to avoid the dissemination of those substances in our environment and to diminish the
pressure on the natural resources, we encourage you to use the appropriate take-back systems.
Those systems will reuse or recycle most of the materials of your end life equipment in a sound
way.
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The crossed-out wheeled bin symbol indicated above invites you to use those systems.
If you need more information on the collection, reuse and recycling systems, please contact
your local or regional waste administration.
9.5 China RoHS
Pb
Hg
Cd
Cr6+
PBB
PBDE
X-Cite TURBO(main unit)
o
o
o
o
o
o
speedDIAL
o
o
o
o
o
o
○：Indicates that this toxic or hazardous substance contained in all of the homogeneous materials
for this part is below the limit requirement in SJ/T11363-2006
x: Indicates that this toxic or hazardous substance contained in at least one of the homogeneous
materials used for this part is above the limit requirement in SJ/T11363-2006 (Enterprises may
further provide in this box technical explanation for marking “X” based on their actual conditions.)
10 Warranty & Repairs
10.1 Warranty Terms
Excelitas warrants the original purchaser for a period of one (1) full year, calculated from the date of
purchase, that the equipment sold is free from defects in material and workmanship. All repairs are
warranted for 90 days. The LED assemblies within the unit have a warranty period of 15,000 hours of
use or 3 years, whichever comes first.
In the event of a claim under this guarantee, the equipment is to be sent postage and carriage paid,
including a description of the fault, to an appropriate Excelitas Authorized Service Center. Returned
equipment will not be received without a Return Material Authorization (RMA) Number, issued by the
Service Center.
In the case of damage caused by wear and tear, careless handling, neglect, by the use of force or in the
case of interventions and repairs not carried out by an Excelitas Service Center, the guarantee ceases to
be valid. This guarantee may not form the basis for any claims for damages, in particular not for
compensation of consequential damages.
The warranty is not transferable. No warranty is extended to perishable items, such as light guides, fuses
and air filters.
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Any claims for units received with defects in material or workmanship must be reported to an
authorized Excelitas Service Center within 30 days from the original date of receipt.
SYSTEM COMPONENT
X-Cite TURBO main unit
X-Cite speedDIAL
LED assembly (each LED)
WARRANTY
1 year
1 year
• 15,000 hrs
• 3 years
whichever
Comes first
WARRANTY VOID IF…
• Unit has been subjected to misuse or
mishandling.
• Unit has been opened or tampered with.
• Unit or cabling has been subjected to misuse
or mishandling.
• Unit has been opened or tampered with.
• LCD display damage (physical).
• LED assembly has been opened or tampered
with.
10.2 Returning equipment to Excelitas
1. Please make a note of the problem encountered, the steps followed to isolate the problem and
the result of any trouble shooting steps taken.
2. Contact the nearest Excelitas Service Center to obtain a Return Material Authorization (RMA)
number. For your convenience, RMA numbers can also be requested on-line at:
www.excelitas.com/Support-UVM
3. Follow shipping instructions provided by the service technician. The unit should be returned in
its original packaging if possible.
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11 Contact Information
11.1 General
Excelitas Canada Inc.
Tel: (905) 821-2600
Fax: (905) 821-2055
Toll Free: 1-800-668-8752 (USA and Canada)
x-cite@excelitas.com
http://www.excelitas.com/x-cite
11.2 Technical Support and Service
For Technical Support and Service specific to Excelitas UV & Microscopy products:
(905) 821-2600, option 3
1-800-668-8752, option 3 (USA and Canada)
www.excelitas.com/Support-UVM
11.3 Accessories and Replacement Parts
Replacement parts and accessories can be purchased directly from Excelitas. For ordering and pricing
information contact the inside sales department at:
(905) 821-2600
1-800-668-8752 (USA and Canada)
x-cite@excelitas.com
http://www.excelitas.com/Pages/Contact/Contact_OX.aspx
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